Species:

Rat

Alternative Name:

150 kDa TGF-beta-1-binding protein, activated T-cell marker CD109, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7, CD109 antigen (Gov platelet alloantigens), CD109 antigen, CD109 molecule, CD109, CPAMD7, CPAMD7r150, DKFZp762L1111, FLJ38569, FLJ41966, Gov platelet alloantigens, p180, Platelet-specific Gov antigen, RP11-525G3.1

Assay Type:

Sandwich

unit:

ng/mL

Standard:

100ng/mL

Sensitivity:

0.94 ng/mL

Detection Range:

1.57-100ng/mL

Assay Time:

3.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL

Research:

Tumor immunity;Hepatology;

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CD109. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CD109 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CD109, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CD109.You can calculate the concentration of Rat CD109 in the samples by comparing the OD of the samples to the standard curve.